This study is part of a project to evaluate and develop metods to control the semen quality in stallions. The study was carried out in cooperation between SLU and Flyinge AB. The objective of the study was to analyse -if there are individual differences in sperm chromatin integrety between different stallions -if there is a correlation between subjectively evaluated sperm motility and sperm chromatin integrity -if sperm chromatin integrity is changed after selection of the semen with a centrifugation method through the silica-spheres, gradient or a single-layer, i.e to use the chromatin integrity analysis as a method to evaluate the selection method. The use of chilled transported semen has increased a lot during the last 10 years, especially among the Swedish warmblood breed. Because the foaling percentage has decreased with the increased use of chilled transported semen, there is a need to develop methods to control the sperm quality. Evaluation of the chromatin integrity could be one way to evaluate the quality of the semen. Chromatin is the DNA in the nucleus of the sperm.

Most Swedish Warmbloods are bred by artificial insemination (AI). Quality control of the semen consists of the subjective evaluation of motility. There is a need for a more accurate and objective method of rating the semen. In this study we compared fluorometry with flowcytometry as a method for evaluating sperm viability. Semen was collected from three stallions, stationed at Markebäcks Gård, Askersund, three times during week 15 and week 34, 2008.

Today, equine breeding is based on performance and conformation, resulting in a vast variation in fertility among different horses, with noticeable economic losses as a consequence. The horse breeding industry is therefore in need of methods that diagnose the spermatozoa concerning their quality and provide the best sperm for AI. The commonly used selection methods for sperm today are: centrifugation, where extender is added, the sample is centrifuged and then resuspended; swim-up self-migration; adherence separation; and density gradient centrifugation. However, none of these methods are in used routinely in practice before inseminating mares with fresh or cooled semen. The aim of this study was to evaluate a new selection method, Single Layer Centrifugation (SLC), and its effect on sperm viability. The viability was analyzed with regard to membrane integrity and membrane stability of ejaculated spermatozoa.

This study is a part of a larger project where the aim has been to evaluate different methods to assure the quality in stallion semen, due to the fact that some stallions in horse breeding have poor semen quality. The increased use of transport semen requires new methods to ensure good semen quality. In this part of the project the viability and concentration in fresh and cooled semen was examined with NucleoCounter SP-100. A comparison between two different commercially available semen extenders, Kenney?s and INRA 96, was made.

Studiens målsättning var att utvärdera kvalitén hos hingstsperma selekterad med hjälp av Single Layer Centrifugation (SLC), med huvudsyftet att studera den mitokondriella membranpotentialen (??m) och förekomsten av reaktiva syreföreningar (ROS) hos hingstspermier före och efter SLC. Ett insemineringsförsök med SLC selekterad och rutinspädd sperma genomfördes även på ett litet antal ston, men inga slutsatser kunde dras av resultaten. Humanstudier har visat att spermier med låg ??m mer frekvent har återfunnits hos män med fertilitetsproblem. Mätning av ??m har gjorts genom att tillsätta den lipofila JC-1, vilket i både studier på både humana- och ekvina spermier har visat sig vara en känslig metod för att särskilja spermier med låg respektive hög ??m.

There is considerable variation between stallions in semen quality and fertility due to the fact that selection of stallions for breeding is not based on fertility or semen quality but on performance. The increasing use of cooled semen for artificial insemination has resulted in decreasing pregnancy rates. Therefore the equine breeding industry needs new methods for evaluation and development of sperm quality and fertility. This study is a part of such a project and is performed by SLU in cooperation with Flyinge AB. Ten stallions at the Flyinge National Stud were used in the study and three ejaculates were collected from each stallion.

This thesis deals with cross-country ski clothing for non-competitive women. Women in this category look for different clothing features in comfort and aesthetics than male top athletes, for whom cross-country skiwear today often seems to be designed. In this thesis the demands of these women have been mapped out by an interview with a reference group of users. The ergonomics of exercising in the winter outdoors are naturally a base for the collection. The collection consists of three functional layers of garments.

Denna studie undersökte om så kallad ?Single Layer Centrifugation? (SLC) genom en silanöverdragen silikat kan selektera spermier med bättre kvalitet. SLC är en metod för att separera spermier med bra kvalitet från andra celler och seminalplasma genom att utnyttja deras olika densitet.Egenskaperna hos spermier varierar mellan arter och därför även möjligheterna att spara och förvara spermier. Utvärdering av spermiekvalitet är viktigt för att förutsäga den fertila förmågan och även utvärdera påverkan de olika processerna som spermierna utsätts för. I denna studie har spermiernas morfologi, motilitet, membranintegritet och akrosomintegritet undersöktes.Ejakulat från sex hundar av olika ras och ålder användes.

Denna studie undersökte om så kallad ?Single Layer Centrifugation? (SLC) genom en silanöverdragen silikat kan selektera spermier med bättre kvalitet. SLC är en metod för att separera spermier med bra kvalitet från andra celler och seminalplasma genom att utnyttja deras olika densitet.Egenskaperna hos spermier varierar mellan arter och därför även möjligheterna att spara och förvara spermier. Utvärdering av spermiekvalitet är viktigt för att förutsäga den fertila förmågan och även utvärdera påverkan de olika processerna som spermierna utsätts för. I denna studie har spermiernas morfologi, motilitet, membranintegritet och akrosomintegritet undersöktes.Ejakulat från sex hundar av olika ras och ålder användes.

A new theory for predicting the windprofile over a canopy has been evaluated. The theory was first presented by Harman and Finnigan (2007). The theory relies on the forming of a mixing-layer above the canopy, due to different mean wind in and above the canopy. Characteristics from both mixing-layer and Monin Obukhov similarity theory have been used to develop the governingequations that give the wind profile. The theory has been used to calculate wind profiles for sixdifferent atmospheric stabilities.

Modeling and simulation is growing ever more important in the development of new products. This thesis describes the use of Hopsan for hydraulic modeling and its use in conjunction with Simulink with the intent of using the model in a hardware-in-the-loop setup. A sensor layer has been created in Simulink to emulate all the internal sensors in a modern forklift. The details of using legacy C-code instead of a hardware MCU for a fully simulated environment, software-in-the-loop has been outlined. There are two major routes one can follow implementing software-in-the-loop, exporting the C-functions to CAPL via a export layer or creating an s-function in Simulink.

At SLU, Divison of Reproduction, Dept of Clinical Science, there is an ongoing project on the development and evaluation of new methods for the assessment of stallion semen quality. This EEF-study, which is a part of that larger project, has focused on the effects on motility and fertility of stallion semen of two different extenders and selection. Both an objective method and a subjective method for analysis have been used and compared in the study. The increase in the use of artificial insemination with cooled semen in Swedish horse breeding over the last decade is thought to play a part in declining foaling rates (Kareskoski et al, 2006, Kuisma et al, 2006). This calls for better evaluation methods of semen quality since cooling and transportation put higher demands on semen quality in order to maintain fertility (Aurich, 2005).

Arbetet behandlar Single Sign-On och två-faktorsautentisering inom hälso- och sjukvården. Syftet med rapporten är att undersöka eventuella utmaningar samt de möjligheter som ges vid införandet av Single Sign-On och två-faktorsautentisering i förhållande till nuvarande autentiseringsmetod. För att besvara rapportens forskningsfråga har kvalitativa forskningsintervjuer tillämpats. Även en enkätundersökning har genomförts för att få användarnas åsikter. Resultatet påvisar att en Single Sign-On tillsammans med två-faktorsautentisering uppfyller de säkerhetskrav som ställs mot hälso- och sjukvården samtidigt som det dagliga arbetet underlättas för vårdpersonalen.

This thesis, designed as a case study, is about complications concerning conservation of a decorative oil paint on lime plaster from the drawing-room in Mostorp castle sited in the county of Halland in south-west of Sweden. The building was built 1878-80 with an exterior of granite. The interior walls were made of clay bricks, lime mortar and lime plaster. The oil paint is applied to a layer of putty. The combination of different materials, which are organic and inorganic, affects the movement caused by internal and external stress.

Today, some kind of laboratory results is required for around 70% of the diagnostics and follow-ups for diseases. In many of the cases the time from sampling to a result is very critical. Therefore the discussion of how to improve this situation has begun. For many analyses serum has been the routine choice for a long time but now it is disputed. After blood collection in a serum tube it is essential to wait 30-60 minutes before centrifugation and analysis of the sample, a long time for someone in an acute state.